DENSITY AND DIVERSITY ESTIMATION OF AMYLOLYTIC BACTERIA AND MOLDS FROM RHIZOSFER AND NON RHIZOSFER SOILS IN BRAWIJAYA UNIVERSITY, MALANG

DENSITY AND DIVERSITY ESTIMATION OF AMYLOLYTIC BACTERIA AND MOLDS FROM RHIZOSFER AND NON RHIZOSFER SOILS IN BRAWIJAYA UNIVERSITY, MALANG

Turhadi (115090113111005)

Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University

ABSTRACT

Amylolytic bacteria or molds are bacteria or molds that capable to produce amylase to breaks starch. This microorganism can found in rhizosphere soil. One way to determine the density and diversity  microorganism are isolation, Total Plate Count (TPC), and iodin assay. The samples that use in this lab work are rhizosphere soil from MIPA forest and non- rhizosphere soil from around UB gate Panjaitan, Malang. The purpose of this lab work are to determine the density and diversity of amylolytic microorganisms (bacteria and molds) in rhizosphere and non- rhizosphere soil. The results are density of amylolytic microorganism in rhizosphere higher than non- rhizosphere soil. In rhizosphere soil found three bacteria isolate and three molds isolate. In non- rhizosphere soil found four bacteria isolate and one mold isolate. Bacteria  diversity index in rhizosphere soil is lower than non- rhizosphere  soil, but molds diversity index is higher than non- rhizosphere soil. We concluded are the difference from density and diversity of amylolytic microorganism because abiotic factors (such as: pH, temperature, organic materials, soil humidity) also different.

Key words: amylolytic, bacteria, molds, non rhizosphere, rhizosphere.